Analysis of Ile aminoacyl synthetase.

Analysis performed on 2024-09-30 10:20:32

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 48581 3541 45040 26217
Archaea 3472 263 3209 1409
Vertebrata 1634 654 980 396
Mammals 587 92 495 133

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
1262 20 1082

Domains

Domains
Current Study 1073, 1263
Guo et al (2010) 1065, 1262

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 1071 191 1262 5.607330
Number of Variants 942 160 1102 5.887500
Number of Pathogenic Variants 17 3 20 5.666667
Number of Benign/Unknown Variants 925 157 1082 5.891720
Fisher exact test 1.000000

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 1064 198 1262 5.373737
Number of Variants 940 162 1102 5.802469
Number of Pathogenic Variants 17 3 20 5.666667
Number of Benign/Unknown Variants 923 159 1082 5.805031
Fisher exact test 1.000000

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 1071 191 0.86 (0.00) 0.79 (0.01) 8.792e-01 8.276e-01 128397.00 9.07e-09
Guo et al (2010) 1064 198 0.86 (0.00) 0.79 (0.01) 8.792e-01 8.283e-01 131603.00 1.21e-08

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains